Effects of dynamic exercise intensity on the activation of hormone-sensitive lipase in human skeletal muscle

Abstract

It has been proposed that hormone-sensitive lipase (HSL) regulates intramuscular triacylglycerol hydrolysis in skeletal muscle. The primary purpose of this study was to examine the early activation of HSL and the changes in the putative intramuscular and hormonal regulators of HSL activity at various aerobic exercise intensities. Eight male subjects cycled for 10 min at power outputs corresponding to 30, 60 and 90% peak oxygen uptake (VO2,peak). Muscle samples were obtained at rest and following 1 and 10 min of exercise. Intramuscular triacylglycerol (mean ± S.E.M.: 24.3 ± 2.3 mmol (kg dry mass (DM))-1), long-chain fatty acyl CoA (13.9 ± 1.4 μmol (kg DM)-1) and HSL activity (1.87 ± 0.07 mmol..