Tadpole-like Conformations of Huntingtin Exon 1 Are Characterized by Conformational Heterogeneity that Persists regardless of Polyglutamine Length
Estella A Newcombe, Kiersten M Ruff, Ashish Sethi, Angelique R Ormsby, Yasmin M Ramdzan, Archa Fox, Anthony W Purcell, Paul R Gooley, Rohit Pappu, Danny M Hatters
Journal of Molecular Biology | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2018
Awarded by US National Institutes of Health
The US National Institutes of Health supported this work through Grant 5R01NS056114 to R.V.P. We thank Oded Kleifeld from the Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, for technical assistance with the proteomics work (now at Faculty of Biology, Technion-lsrael Institute of Technology, Haifa 3200003). This work builds on a prior collaboration with John Warner IV, Piau Siong Tan, Edward Lemke, and Hilal Lashuel that yielded atomistic descriptions of Httexl monomers using smFRET and computer simulations . We (K.M. R. and R.V.P) are grateful to Drs. Warner, Lemke, and Lashuel for their assistance and for their insights regarding the conformational ensembles of Httex1.