Journal article

Human plasma proteome association and cytotoxicity of nano-graphene oxide grafted with stealth polyethylene glycol and poly(2-ethyl-2oxazoline)

Miaoyi Wang, Ove JR Gustafsson, Ghizal Siddiqui, Ibrahim Javed, Hannah G Kelly, Thomas Blin, Hong Yin, Stephen J Kent, Darren J Creek, Kristian Kempe, Pu Chun Ke, Thomas P Davis

NANOSCALE | ROYAL SOC CHEMISTRY | Published : 2018

Abstract

Polyethylene glycol (PEG) is a gold standard against protein fouling. However, recent studies have revealed surprising adverse effects of PEG, namely its immunogenicity and shortened bio-circulation upon repeated dosing. This highlights a crucial need to further examine 'stealth' polymers for controlling the protein 'corona', a new challenge in nanomedicine and bionanotechnology. Poly(2-ethyl-2-oxazoline) (PEtOx) is another primary form of stealth polymer that, despite its excellent hydrophilicity and biocompatibility, has found considerably less applications compared with PEG. Herein, we performed label-free proteomics to compare the associations of linear PEG- and PEtOx-grafted nano-graphe..

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Grants

Awarded by ARC


Awarded by NHMRC-ARC Dementia Research Development Fellowship


Funding Acknowledgements

This work was supported by ARC Project CE140100036 (Davis). Davis is thankful for the award of an Australian Laureate Fellowship from the ARC. Kempe gratefully acknowledges the award of an NHMRC-ARC Dementia Research Development Fellowship (APP1109945). The authors thank the blood donors and Joshua Glass for technical assistance.