Journal article
Identification of a Siglec-F granulocyte-macrophage progenitor
JE Bolden, EC Lucas, G Zhou, JA O'Sullivan, CA de Graaf, MD McKenzie, L Di Rago, TM Baldwin, J Shortt, WS Alexander, BS Bochner, ME Ritchie, DJ Hilton, KA Fairfax
Journal of Leukocyte Biology | WILEY | Published : 2018
Abstract
In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b + Siglec-F+ IL5Rα− myeloid po..
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Awarded by Scripps Research Institute
Funding Acknowledgements
We acknowledge Keti Stoev, Merle Dayton, Kim Birchall, and Jessica Martin for sharing their technical expertise. We thank Dr. James Paulson at The Scripps Research Institute (La Jolla, CA, USA) for provision of the 9C7 anti-Siglec-F mAb. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. This work was supported by Early Career Fellowships from the National Health and Medical Research Council of Australia (NHMRC) to J.E.B. (GNT0637403) and C.A.d.G. (GNT1035229), NHMRC project grant to D.J.H. (1048087), NHMRC program grant (1113577) and fellowship (1058344) to W.S.A., Commonwealth Serum Laboratories (CSL) funding to D.J.H., P01HL107151 from the National Heart, Lung, and Blood Institute to B.S.B. and AI072265 from the National Institute of Allergy and Infectious Diseases to B.S.B.