Genome-wide maps of nuclear lamina interactions in single human cells.
Jop Kind, Ludo Pagie, Sandra S de Vries, Leila Nahidiazar, Siddharth S Dey, Magda Bienko, Ye Zhan, Bryan Lajoie, Carolyn A de Graaf, Mario Amendola, Geoffrey Fudenberg, Maxim Imakaev, Leonid A Mirny, Kees Jalink, Job Dekker, Alexander van Oudenaarden, Bas van Steensel
Cell | Published : 2015
Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination..View full abstract