Journal article
An interdigitated electrode biosensor platform for rapid HLA-B*15:02 genotyping for prevention of drug hypersensitivity
GV Soraya, J Chan, TC Nguyen, DH Huynh, CD Abeyrathne, G Chana, M Todaro, E Skafidas, P Kwan
Biosensors and Bioelectronics | ELSEVIER ADVANCED TECHNOLOGY | Published : 2018
Abstract
Prevention of life threatening hypersensitivity reactions to carbamazepine is possible through pre-treatment screening of the associated HLA-B*15:02 risk allele. However, clinical implementation of screening is hindered by the high cost and slow turnaround of conventional HLA typing methods. We have developed an interdigitated electrode (IDE) biosensor platform utilizing loop mediated isothermal amplification (LAMP) that can rapidly detect the HLA-B*15:02 allele. DNA amplification is followed by solid-phase hybridization of LAMP amplicons to a DNA probe immobilized on the IDE sensor surface, resulting in a change in sensor impedance. The testing platform does not require DNA extraction or po..
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Awarded by Tree Research and Education Endowment Fund
Funding Acknowledgements
This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). E.S. thanks the generous support of Sue and Leigh Clifford in establishing the Chair in Neural Engineering. G.V.S. was supported by the Indonesian Endowment Fund for Education (LPDP). This work was supported in part by the Australian Research Council (DP140101967) and the Royal Melbourne Hospital Neuroscience Foundation.