A unique F-19 MRI agent for the tracking of non phagocytic cells in vivo
Shehzahdi S Moonshi, Cheng Zhang, Hui Peng, Simon Puttick, Stephen Rose, Nicholas M Fisk, Kishore Bhakoo, Brett W Stringer, Greg G Qiao, Paul A Gurr, Andrew K Whittaker
NANOSCALE | ROYAL SOC CHEMISTRY | Published : 2018
There is currently intense interest in new methods for understanding the fate of therapeutically-relevant cells, such as mesenchymal stem cells (MSCs). The absence of a confounding background signal and consequent unequivocal assignment makes 19F MRI one of the most attractive modalities for the tracking of injected cells in vivo. We describe here the synthesis of novel partly-fluorinated polymeric nanoparticles with small size and high fluorine content as MRI agents. The polymers, constructed from perfluoropolyether methacrylate (PFPEMA) and oligo(ethylene glycol) methacrylate (OEGMA) have favourable cell uptake profiles and excellent MRI performance. To facilitate cell studies the polymer ..View full abstract
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Awarded by Australian Research Council
Awarded by National Health and Medical Research Council
The authors acknowledge the Australian Research Council (CE140100036, DP0987407, DP110104299, LE0775684, LE0668517, and LE0882357) and the National Health and Medical Research Council (APP1021759) for funding of this research. The Australian National Fabrication Facility, Queensland Node, is also acknowledged for access to some items of equipment. We thank Dr Rebecca Pelekanos and Ms Varda Sardesai from UQCCR for their assistance in isolation and characterization of MSCs, Joyce Tang for her aid in characterization of labelled MSCs and Dr Nyoman Kurniawan for assistance with the MRI measurements.