Journal article
Amyloid Precursor Protein Dimerisation Reduces Neurite Outgrowth
L Luu, GD Ciccotosto, LJ Vella, L Cheng, LC Roisman, G Multhaup, AF Hill, LM Munter, R Cappai
Molecular Neurobiology | SPRINGER | Published : 2019
Abstract
The amyloid precursor protein (APP) undergoes extensive metabolism, and its transport and proteolytic processing can be modulated by its ability to form a homodimer. We have investigated the functional consequences of stabilised APP dimer expression in cells by studying the engineered dimerisation of the APP L17C (residue 17 in Aβ sequence) construct, which is associated with a 30% increase in APP dimer expression, on APP’s neurite outgrowth promoting activity. Overexpression of APP L17C in SH-SY5Y cells decreased neurite outgrowth upon retinoic acid differentiation as compared to overexpressing APP WT cells. The APP L17C phenotype was rescued by replacing the APP L17C media with conditioned..
View full abstractRelated Projects (1)
Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported from funding from the Australia National Health and Medical Research Council (R.C., A.F.H.).