Journal article

Differential protein expression and post-translational modifications in metronidazole-resistant Giardia duodenalis

Samantha J Emery, Louise Baker, Brendan RE Ansell, Mehdi Mirzaei, Paul A Haynes, Malcom J McConville, Staffan G Svard, Aaron R Jex



Background: Metronidazole (Mtz) is the frontline drug treatment for multiple anaerobic pathogens, including the gastrointestinal protist, Giardia duodenalis. However, treatment failure is common and linked to in vivo drug resistance. In Giardia, in vitro drug-resistant lines allow controlled experimental interrogation of resistance mechanisms in isogenic cultures. However, resistance-associated changes are inconsistent between lines, phenotypic data are incomplete, and resistance is rarely genetically fixed, highlighted by reversion to sensitivity after drug selection ceases or via passage through the life cycle. Comprehensive quantitative approaches are required to resolve isolate variabili..

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Awarded by Australian Research Council (ARC)

Awarded by NHMRC

Awarded by Jack Brockhoff Foundation Early Career Grant

Funding Acknowledgements

This work, including the efforts of A.J. and M.M., was funded by Australian Research Council (ARC) (LP120200122). S.E., B.A., L.B., and A.J. are supported by the Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme. A.J. is also supported by a NHMRC Career Development Fellowship (APP1126395). S.E. and this research are also supported by a Jack Brockhoff Foundation Early Career Grant (ID 4184). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.