Journal article
Functional characterization of a glycosyltransferase from the moss physcomitrella patens involved in the biosynthesis of a novel cell wall arabinoglucan
AW Roberts, J Lahnstein, YSY Hsieh, X Xing, K Yap, AM Chaves, TR Scavuzzo-Duggan, G Dimitroff, A Lonsdale, E Roberts, V Bulone, GB Fincher, MS Doblin, A Bacic, RA Burton
Plant Cell | AMER SOC PLANT BIOLOGISTS | Published : 2018
DOI: 10.1105/tpc.18.00082
Abstract
Mixed-linkage (1,3;1,4)-β-glucan (MLG), an abundant cell wall polysaccharide in the Poaceae, has been detected in ascomycetes, algae, and seedless vascular plants, but not in eudicots. Although MLG has not been reported in bryophytes, a predicted glycosyltransferase from the moss Physcomitrella patens (Pp3c12_24670) is similar to a bona fide ascomycete MLG synthase. We tested whether Pp3c12_24670 encodes an MLG synthase by expressing it in wild tobacco (Nicotiana benthamiana) and testing for release of diagnostic oligosaccharides from the cell walls by either lichenase or (1,4)-β-glucan endohydrolase. Lichenase, an MLG-specific endohydrolase, showed no activity against cell walls from transf..
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Awarded by National Science Foundation
Funding Acknowledgements
This work was supported by National Science Foundation Award IOS-1257047 and the Australian Research Council Centre for Excellence in Plant Cell Walls Grant CE1101007. DNA sequencing was conducted at a Rhode Island NSF EPSCoR research facility, the Genomics and Sequencing Center, supported in part by the National Science Foundation EPSCoR Cooperative Agreement EPS-1004057. Oligosaccharide linkage analysis was supported by the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy Grant DE-FG02-93ER20097 to Parastoo Azadi at the Complex Carbohydrate Research Center. We thank Luis Vidali for his gift of the YFP:GmMan Golgi marker line of P. patens and Magdalena Bezanilla for the gift of the mEGFP entry clone.