Journal article

Mix-and-diffuse serial synchrotron crystallography

Kenneth R Beyerlein, Dennis Dierksmeyer, Valerio Mariani, Manuela Kuhn, Iosifina Sarrou, Angelica Ottaviano, Salah Awel, Juraj Knoska, Silje Fuglerud, Olof Jonsson, Stephan Stern, Max O Wiedorn, Oleksandr Yefanov, Luigi Adriano, Richard Bean, Anja Burkhardt, Pontus Fischer, Michael Heymann, Daniel A Horke, Katharina EJ Jungnickel Show all

IUCrJ | INT UNION CRYSTALLOGRAPHY | Published : 2017

Abstract

Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotrio..

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University of Melbourne Researchers

Grants

Awarded by European Research Council under the European Union's Seventh Framework Programme (FP7) through the Consolidator Grant COMOTION


Awarded by excellence cluster 'The Hamburg Center for Ultrafast Imaging - Structure, Dynamics and Control of Matter at the Atomic Scale' of the Deutsche Forschungsgemeinschaft (CUI)


Funding Acknowledgements

This work was supported by the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013) through the Consolidator Grant COMOTION (ERC-614507-Kupper), by the excellence cluster 'The Hamburg Center for Ultrafast Imaging - Structure, Dynamics and Control of Matter at the Atomic Scale' of the Deutsche Forschungsgemeinschaft (CUI, DFG-EXC1074) and by the Helmholtz Gemeinschaft through the 'Impuls- und Vernetzungsfond'.