Journal article

Questionable 16S ribosomal RNA gene annotations are frequent in completed microbial genomes.

Yu-Hsiang Lin, Bill CH Chang, Pei-Wen Chiang, Sen-Lin Tang

Gene | Published : 2008

Abstract

According to recent reports, many ribosomal RNA gene annotations are still questionable, and the use of inappropriate tools for annotation has been blamed. However, we believe that the abundant 16S rRNA partial sequence in the databases, mainly created by culture-independent PCR methods, is another main cause of the ambiguous annotations of 16S rRNA. To examine the current status of 16S rRNA gene annotations in complete microbial genomes, we used as a criterion the conserved anti-SD sequence, located at the 3' end of the 16S rRNA gene, which is commonly overlooked by culture-independent PCR methods. In our large survey, 859 16S rRNA gene sequences from 252 different species of the microbial ..

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University of Melbourne Researchers