Journal article

Sequential low and medium frequency ultrasound assists biodegradation of wheat chaff by white rot fungal enzymes

Christine M Oliver, Raymond Mawson, Laurence D Melton, Geoff Dumsday, Jessica Welch, Peerasak Sanguansri, Tanoj K Singh, Mary Ann Augustin

Carbohydrate Polymers | ELSEVIER SCI LTD | Published : 2014

Abstract

The consequences of ultrasonic pre-treatment using low (40 kHz) and medium (270 kHz) frequency (40 kHz followed by 270 kHz) on the degradation of wheat chaff (8 g 100ml(-1) acetate buffer, pH 5) were evaluated. In addition, the effects of the ultrasonic pre-treatment on the degradation of the wheat chaff when subsequently exposed to enzyme extracts from two white rot fungi (Phanerochaete chrysosporium and Trametes sp.) were investigated. Pre-treatment by sequential low and medium frequency ultrasound had a disruptive effect on the lignocellulosic matrix. Analysis of the phenolic-derived volatiles after enzymatic hydrolysis showed that biodegradation with the enzyme extract obtained from P. c..

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University of Melbourne Researchers