Structural Analysis of Polysaccharide Networks by Transmission Electron Microscopy: Comparison with Small-Angle X-ray Scattering
Pablo Hernandez-Cerdan, Bradley W Mansel, Andrew Leis, Leif Lundin, Martin AK Williams
Biomacromolecules | AMER CHEMICAL SOC | Published : 2018
Polysaccharide gels assembled from the anionic biopolymers pectin and carrageenan have been studied using transmission electron microscopy (TEM). Gels were formed in several different ways: for pectin, hydrogen bonding was used to form junction zones between strands, whereas for carrageenan systems, several different ion types were used to form ionotropic networks. Using this approach, several distinct network architectures were realized. In addition to preparing gelled samples for electron microscopy, a set of samples was taken without performing the additional treatment necessitated by the TEM measurements, and these were studied directly by small-angle X-ray scattering (SAXS). Taking care..View full abstract
The authors acknowledge the New Zealand Synchrotron group for funding for the SAXS experiments and the SAXS beam-line scientists at the Australian Synchrotron for assistance. The MacDiarmid Institute is thanked for PhD studentships (P.H.-C., B.W.M.), and Raffaele Mezzenga, Niklas Loren and Annika Altskas for interesting discussions.