F-19 NMR studies provide insights into lipid membrane interactions of listeriolysin O, a pore forming toxin from Listeria monocytogenes
Mirijam Kozorog, Marc-Antoine Sani, Martina Lenarcic Zivkovic, Gregor Ilc, Vesna Hodnik, Frances Separovic, Janez Plavec, Gregor Anderluh
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2018
Listeria monocytogenes is a mammalian pathogen that causes gastroenteritis, miscarriages and infections of the central nervous system in immunocompromised individuals. Its main virulence factor is listeriolysin O (LLO), a pore-forming cholesterol-dependent cytolysin (CDC), which enables bacterial escape from the phagolysosome and contributes to bacterial pathogenicity. Details of cholesterol (Chol) recognition and membrane binding mechanisms by LLO are still not known. Here we used 19F-NMR spectroscopy in order to assess LLO-Chol interactions in solution and in a Chol-rich membrane environment. LLO has six tryptophan residues located in the region of the molecule that is first in contact wit..View full abstract
Awarded by Slovenian Research Agency
The work presented in this paper was supported by the program grant Molecular Interactions (P1-0391) from the Slovenian Research Agency, EN-FIST Centre of Excellence, Slovene Human Resources Development and Scholarship Fund and Australian Research Council LIEF support for solid-state NMR. We would like to thank Neza Omersa for the help with the SPR experiment.