The Role of the Microglial Cx3crl Pathway in the Postnatal Maturation of Retinal Photoreceptors
Andrew I Jobling, Michelle Waugh, Kirstan A Vessey, Joanna A Phipps, Lidia Trogrlic, Una Greferath, Samuel A Mills, Zhi L Tan, Michelle M Ward, Erica L Fletcher
Journal of Neuroscience | SOC NEUROSCIENCE | Published : 2018
Microglia are the resident immune cells of the CNS, and their response to infection, injury and disease is well documented. More recently, microglia have been shown to play a role in normal CNS development, with the fractalkine-Cx3cr1 signaling pathway of particular importance. This work describes the interaction between the light-sensitive photoreceptors and microglia during eye opening, a time of postnatal photoreceptor maturation. Genetic removal of Cx3cr1 (Cx3cr1GFP/GFP ) led to an early retinal dysfunction soon after eye opening [postnatal day 17 (P17)] and cone photoreceptor loss (P30 onward) in mice of either sex. This dysfunction occurred at a time when fractalkine expression was pre..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This work was supported by the National Health and Medical Research Council of Australia (Grant #1061418 to E.L.F. and A.I.J.; Grant #1061419 to E.L.F. and K.A.V.) and the Victorian Science Agenda (to E.L.F.). We thank Gene Venables for processing and imaging the acetylated alpha-tubulin, and Horace (Wing Hei) Chan for help with the photoreceptor cilium diagram. We also thank Associate Professor Paulo Ferreira from Duke University Medical Center (Durham, NC) and Dr. Tiansen Li from the National Eye Institute (Bethesda, MD) for the donation of antibodies to probe the photoreceptor cilium.