Journal article
Mucosal-associated invariant T cells are depleted and exhibit altered chemokine receptor expression and elevated granulocyte macrophage-colony stimulating factor production during end-stage renal disease
JA Juno, JLM Waruk, KM Wragg, C Mesa, C Lopez, J Bueti, SJ Kent, TB Ball, SA Kiazyk
Frontiers in Immunology | FRONTIERS MEDIA SA | Published : 2018
Abstract
Background: End-stage renal disease (ESRD) is associated with an increased susceptibility to infectious diseases, including infection with Mycobacterium tuberculosis (Mtb). Mucosal-associated invariant T (MAIT) cells recognize vitamin B metabolites produced by many bacterial species, including Mtb, and may play an important role in providing protective immunity against tuberculosis infection in the lung. To date, little is known about MAIT cell frequency, phenotype, or function in ESRD patients. Methods: MAIT cells, identified by surface marker expression or MR1 tetramer binding, were characterized in 20 ESRD and 20 healthy control participants by multicolor flow cytometry. Ex vivo MAIT cell..
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Awarded by National Institutes of Health
Funding Acknowledgements
The authors would like to thank the study participants and clinic staff for their participation in the research. Additionally, we thank Sidonia Eckle, Alexandra Corbett, and Bronwyn Meehan for technical assistance producing the human MR1 tetramer. The following reagent was obtained through the AIDS Reagent Program, Division of AIDS, NIAID, NIH: anti-human alpha 4-beta 7 integrin (Act1) (cat#11718) from Dr. A. A. Ansari. JJ is funded by a fellowship from the National Health and Medical Research Council (NHMRC). The study was funded by the Public Health Agency of Canada.