Outer Membrane Vesicle Proteome of Porphyromonas gingivalis Is Differentially Modulated Relative to the Outer Membrane in Response to Heme Availability
Paul D Veith, Caroline Luong, Kheng H Tan, Stuart G Dashper, Eric C Reynolds
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2018
Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme, which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analyzed by tandem mass spectrometry and MaxQuant label-free quantification. In total, 1211 proteins were quantified, with 108 and 49 proteins significantly changing..View full abstract
Awarded by Australian Government Department of Industry, Innovation and Science
This work was supported by the Australian Government Department of Industry, Innovation and Science Grant ID 20080108. We thank Dr. Shuai Nie, Dr. Ching-Seng Ang, and Dr. Nicholas Williamson for the acquisition of Orbitrap LC MS/MS data within the Mass Spectrometry and Proteomics Facility at the Bio21 Institute, The University of Melbourne, Australia.