Comparison of five methods for the estimation of methane production from vented in vitro systems
Pablo S Alvarez Hess, Richard J Eckard, Joe L Jacobs, Murray C Hannah, Peter J Moate
Journal of the Science of Food and Agriculture | Wiley | Published : 2019
BACKGROUND There are several methods for estimating methane production (MP) from feedstuffs in vented in vitro systems. One method (A; ‘gold standard’) measures methane proportions in the incubation bottle's headspace (HS) and in the vented gas collected in gas bags. Four other methods (B, C, D and E) measure methane proportion in a single gas sample from the HS. Method B assumes the same methane proportion in the vented gas as in the HS, method C assumes constant methane to carbon dioxide ratio, method D has been developed based on empirical data, and method E assumes constant individual venting volumes. This study aimed to compare the MP predictions from these methods to that of the gold s..View full abstract
This project was supported by Emissions Reduction Alberta, Canada, the University of Melbourne, Agriculture Victoria and the Primary Industries Climate Challenges Centre.