Journal article

Elastomeric microvalve geometry affects haemocompatibility

Crispin Szydzik, Rose J Brazilek, Khashayar Khoshmanesh, Farzan Akbaridoust, Markus Knoerzer, Peter Thurgood, Ineke Muir, Ivan Marusic, Harshal Nandurkar, Arnan Mitchell, Warwick S Nesbitt

LAB ON A CHIP | ROYAL SOC CHEMISTRY | Published : 2018

Abstract

This paper reports on the parameters that determine the haemocompatibility of elastomeric microvalves for blood handling in microfluidic systems. Using a comprehensive investigation of blood function, we describe a hierarchy of haemocompatibility as a function of microvalve geometry and identify a "normally-closed" v-gate pneumatic microvalve design that minimally affects blood plasma fibrinogen and von Willebrand factor composition, minimises effects on erythrocyte structure and function, and limits effects on platelet activation and aggregation, while facilitating rapid switching control for blood sample delivery. We propose that the haemodynamic profile of valve gate geometries is a signi..

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Grants

Funding Acknowledgements

We would like to acknowledge Robert Andrews, Justin Hamilton, and Elizabeth Gardner for helpful discussions and technical advice. We thank the staff and students at the Australian Centre for Blood Diseases for help with blood collection. The authors acknowledge the facilities and expertise of Monash Micro Imaging (MMI), Monash University, CSL Ltd, The Department of Clinical Haematology - Alfred Hospital, and Monash Statistical Services - Alfred Hospital. Devices were fabricated at the Micro Nano Research Facility (MNRF) - RMIT University. CS is a recipient of a Professor Robert and Josephine Shanks Scholarship. RJB is a recipient of an Australian Research Training Program Scholarship. WSN is a recipient of an RMIT Vice Chancellor's Senior Research Fellowship.