Journal article

The early in-vivo effects of a single anti-emetic dose of dexamethasone on innate immune cell gene expression and activation in healthy volunteers

CR Bain, DF Draxler, R Taylor, S Wallace, O Gouldthorpe, TB Corcoran, PS Myles, RL Medcalf, K Bozaoglu

Anaesthesia | WILEY | Published : 2018

Abstract

Dexamethasone is often administered to surgical patients for anti-emetic prophylaxis. This study examined the early (up to 24 h) in-vivo effects of dexamethasone (8 mg) to demonstrate the magnitude and temporal nature of changes on circulating peripheral blood mononuclear cell gene expression and activation in 10 healthy male volunteers. Blood samples were drawn at baseline, 2 h, 4 h and 24 h. Gene expression was measured using quantitative real-time polymerase chain reaction. Cytokine expression was measured using multiplex immuno-assays. Innate immune cell phenotypes were examined with flow cytometry. Dexamethasone resulted in rapid transient changes in immunophilin (p = 0.0247), plasminog..

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Grants

Awarded by Australian and New Zealand College of Anaesthetists (ANZCA) pilot grant funds


Awarded by Australian National Health and a Medical Research Council (NHMRC)


Funding Acknowledgements

Funding kindly provided by the Australian and New Zealand College of Anaesthetists (ANZCA) pilot grant funds (Study no p15008), Australian National Health and a Medical Research Council (NHMRC) Project Grant (APP1020284). No competing interests declared.