Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis
Md Saruar Bhuiyan, Felix Ellett, Gerald L Murray, Xenia Kostoulias, Gustavo M Cerqueira, Keith E Schulze, Mohd Hafidz Mahamad Maifiah, Jian Li, Darren J Creek, Graham J Lieschke, Anton Y Peleg
Proceedings of the National Academy of Sciences | NATL ACAD SCIENCES | Published : 2016
Innate cellular immune responses are a critical first-line defense against invading bacterial pathogens. Leukocyte migration from the bloodstream to a site of infection is mediated by chemotactic factors that are often host-derived. More recently, there has been a greater appreciation of the importance of bacterial factors driving neutrophil movement during infection. Here, we describe the development of a zebrafish infection model to study Acinetobacter baumannii pathogenesis. By using isogenic A. baumannii mutants lacking expression of virulence effector proteins, we demonstrated that bacterial drivers of disease severity are conserved between zebrafish and mammals. By using transgenic zeb..View full abstract
We thank Dr. Connie Wong and Alyce Nicholls for supporting the chemotaxis assay; Monash Micro Imaging for use of the imaging facilities; Monash FishCore facilities for the care and maintenance of zebrafish; and Max Cryle and Matthew Belousoff for assistance with the metabolomics assays. This work was supported by an Australian National Health and Medical Research Council (NHMRC) Career Development fellowship (to A.Y.P. and D.J.C.), and an Australian Leadership Award by the Australian government (to M.S.B.). G.J.L. is an NHMRC Senior Research Fellow. The Australian Regenerative Medicine Institute is supported by funds from the State Government of Victoria and the Australian Federal Government.