Journal article
Parathyroid hormone-related protein negatively regulates tumor cell dormancy genes in a PTHR1/cyclic AMP-independent manner
RW Johnson, Y Sun, PWM Ho, ASM Chan, JA Johnson, NJ Pavlos, NA Sims, TJ Martin
Frontiers in Endocrinology | FRONTIERS MEDIA SA | Published : 2018
Abstract
Parathyroid hormone-related protein (PTHrP) expression in breast cancer is enriched in bone metastases compared to primary tumors. Human MCF7 breast cancer cells "home" to the bones of immune deficient mice following intracardiac inoculation, but do not grow well and stain negatively for Ki67, thus serving as a model of breast cancer dormancy in vivo. We have previously shown that PTHrP overexpression in MCF7 cells overcomes this dormant phenotype, causing them to grow as osteolytic deposits, and that PTHrP-overexpressing MCF7 cells showed significantly lower expression of genes associated with dormancy compared to vector controls. Since early work showed a lack of cyclic AMP (cAMP) response..
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Awarded by Center for Outcomes Research and Evaluation, Yale School of Medicine
Funding Acknowledgements
A portion of these data were previously published as a conference paper supplement (41). The authors wish to acknowledge the expert technical support of the VANGARD core facilities. RJ is supported in part by NIH award R00CA194198 (RJ). Experiments performed at Vanderbilt were supported in part by scholarship funds from NIH award P30CA068485 Vanderbilt-Ingram Cancer Center Support Grant. Experiments performed at SVI were supported in part by NHMRC grant 1081242 to NS and TM, and SVI receives support from the Victorian Government OIS Program. AC was supported in part by an Australian and New Zealand Bone and Mineral Society Christine & T. Jack Martin Research Travel Grant and NHMRC grant 1078280 to NP.