Journal article
Individuals with HIV-1 Subtype C Infection and Cryptococcal Meningitis Exhibit Viral Genetic Intermixing of HIV-1 between Plasma and Cerebrospinal Fluid and a High Prevalence of CXCR4-Using Variants
K Sojane, RT Kangethe, CC Chang, MYS Moosa, SR Lewin, MA French, T Ndung'U
AIDS Research and Human Retroviruses | MARY ANN LIEBERT, INC | Published : 2018
Abstract
The genotypic properties of human immunodeficiency virus type 1 (HIV-1) subtype C in individuals presenting with cryptococcal meningitis (CM) are not well established. Employing single-genome amplification as well as bulk PCR, cloning and sequencing strategies, we evaluated the genetic properties of HIV-1 subtype C env in 16 antiretroviral therapy-naive study participants with CM. Eleven of the 16 participants had matched blood plasma and cerebrospinal fluid (CSF) evaluated, with the rest having either a plasma or CSF sample evaluated. Before antiretroviral therapy initiation, matched plasma and CSF-derived env sequences of all 11 participants displayed genetic intermixing between the two co..
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Awarded by Howard Hughes Medical Institute
Funding Acknowledgements
This research was funded by the Howard Hughes Medical Institute, the South African Department of Science and Technology/National Research Foundation Research Chairs Initiative and the Victor Daitz Foundation. Partial funding was provided by the Sub-Saharan African Network for TB/HIV Research Excellence (SANTHE), a DELTAS Africa Initiative [grant # DEL-15-006]. The DELTAS Africa Initiative is an independent funding scheme of the African Academy of Sciences (AAS) Alliance for Accelerating Excellence in Science in Africa (AESA) and supported by the New Partnership for Africa's Development Planning and Coordinating Agency (NEPAD Agency), with funding from the Wellcome Trust [grant # 107752/Z/15/Z] and the UK government. The views expressed in this publication are those of the author(s) and not necessarily those of AAS, NEPAD Agency, Wellcome Trust, or the UK government. We extend our gratitude to Professor Paul Gorry, head of the HIV Molecular Pathogenesis Laboratory (Burnet Institute), for providing us with the pSVIII-Env plasmid. Finally, we thank and acknowledge the study participants.