Journal article
Macrophages protect Talaromyces marneffei conidia from myeloperoxidase-dependent neutrophil fungicidal activity during infection establishment in vivo
F Ellett, V Pazhakh, L Pase, EL Benard, H Weerasinghe, D Azabdaftari, S Alasmari, A Andrianopoulos, GJ Lieschke
Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2018
Abstract
Neutrophils and macrophages provide the first line of cellular defence against pathogens once physical barriers are breached, but can play very different roles for each specific pathogen. This is particularly so for fungal pathogens, which can occupy several niches in the host. We developed an infection model of talaromycosis in zebrafish embryos with the thermally-dimorphic intracellular fungal pathogen Talaromyces marneffei and used it to define different roles of neutrophils and macrophages in infection establishment. This system models opportunistic human infection prevalent in HIV-infected patients, as zebrafish embryos have intact innate immunity but, like HIV-infected talaromycosis pa..
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Awarded by Department of Industry, Innovation, Science, Research and Tertiary Education, Australian Government
Funding Acknowledgements
Grant support is as follows: GJL, AA, National Health and Medical Research Council (461208, 637394, 1044754, 1069284); FE and LP, Australian Postgraduate Award, Walter and Eliza Hall Institute Edith Moffatt Scholarship; FE, Monash University Bridging Postdoctoral Fellowship, and his work in the laboratory of Dr D. Irimia was supported by the MGH BioMEMS Center (NIH grant EB002503); VP, Monash Graduate Scholarship, Monash International Postgraduate Research Scholarship, Monash Postgraduate Publication Award. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.