Journal article
Gestational diabetes mellitus modulates cholesterol homeostasis in human fetoplacental endothelium
Y Sun, S Kopp, J Strutz, CC Gali, M Zandl-Lang, E Fanaee-Danesh, A Kirsch, S Cvitic, S Frank, R Saffery, I Björkhem, G Desoye, C Wadsack, U Panzenboeck
Biochimica Et Biophysica Acta Molecular and Cell Biology of Lipids | ELSEVIER SCIENCE BV | Published : 2018
Abstract
Gestational diabetes mellitus (GDM) is associated with excessive oxidative stress which may affect placental vascular function. Cholesterol homeostasis is crucial for maintaining fetoplacental endothelial function. We aimed to investigate whether and how GDM affects cholesterol metabolism in human fetoplacental endothelial cells (HPEC). HPEC were isolated from fetal term placental arterial vessels of GDM or control subjects. Cellular reactive oxygen species (ROS) were detected by H 2 DCFDA fluorescent dye. Oxysterols were quantified by gas chromatography–mass spectrometry analysis. Genes and proteins involved in cholesterol homeostasis were detected by real-time PCR and immunoblotting, respe..
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Awarded by Austrian Science Fund
Funding Acknowledgements
We thank statistician BA. Katharina Eberhard (Medical University of Graz) for assisting statistical analysis and Prof. Kerry-Anne Rye (University of New South Wales) for valuable suggestions on the manuscript. This work was supported by the PhD program Molecular Medicine by the Medical University of Graz (C.W., G.D., U.P.), and the Austrian Science Fund, grants DK-MCD W1226-B18 (to U.P.) and P24783 (to U.P.).