Journal article
Plasmacytoid dendritic cell heterogeneity is defined by CXCL10 expression following TLR7 stimulation
C Marsman, F Lafouresse, Y Liao, TM Baldwin, LA Mielke, Y Hu, M Mack, PJ Hertzog, CA de Graaf, W Shi, JR Groom
Immunology and Cell Biology | WILEY | Published : 2018
DOI: 10.1111/imcb.12173
Abstract
Plasmacytoid dendritic cells (pDCs) play a critical role in bridging the innate and adaptive immune systems. pDCs are specialized type I interferon (IFN) producers, which has implicated them as initiators of autoimmune pathogenesis. However, little is known about the downstream effectors of type I IFN signaling that amplify autoimmune responses. Here, we have used a chemokine reporter mouse to determine the CXCR3 ligand responses in DCs subsets. Following TLR7 stimulation, conventional type 1 and type 2 DCs (cDC1 and cDC2, respectively) uniformly upregulate CXCL10. By contrast, the proportion of chemokine positive pDCs was significantly less, and stable CXCL10+ and CXCL10− populations could ..
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Awarded by CSL Behring
Funding Acknowledgements
We thank Ken D Shortman, Stephen L Nutt and Gabrielle T Belz for helpful discussion and critical reading of the manuscript. We thank Angela D'Amico and members of the Groom lab for technical assistance. JRG is supported by Australian Research Council Future Fellowship (FT130100708). FL and WS are supported by a Walter and Eliza Hall Centenary Fellowship sponsored by CSL. CAdG is supported by an National Health and Medical Research Council Early Career Fellowship (GNT1035229). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.