Cellulose synthase complexes display distinct dynamic behaviors during xylem transdifferentiation
Yoichiro Watanabe, Rene Schneider, Sarah Barkwill, Eliana Gonzales-Vigil, Joseph L Hill, A Lacey Samuels, Staffan Persson, Shawn D Mansfield
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2018
In plants, plasma membrane-embedded CELLULOSE SYNTHASE (CESA) enzyme complexes deposit cellulose polymers into the developing cell wall. Cellulose synthesis requires two different sets of CESA complexes that are active during cell expansion and secondary cell wall thickening, respectively. Hence, developing xylem cells, which first undergo cell expansion and subsequently deposit thick secondary walls, need to completely reorganize their CESA complexes from primary wall- to secondary wall-specific CESAs. Using live-cell imaging, we analyzed the principles underlying this remodeling. At the onset of secondary wall synthesis, the primary wall CESAs ceased to be delivered to the plasma membrane ..View full abstract
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Awarded by Australian Research Council Future Fellowship
Awarded by Department of Energy Office of Sciences, Basic Energy Sciences
We thank the University of British Columbia Bioimaging Facility and the Biological Optical Microscopy Platform at the University of Melbourne for technical support; Dr. Ming Tien (Pennsylvania State University) for the gift of the CESA antibodies; and Dr. Taku Demura (Nara Institute of Science and Technology) for seeds of the 355::VND7-VP16-GRinducible system. S.D.M. and A.L.S. received funding support from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grants program and the NSERC Working on Walls CREATE program. S.P. was funded through Australian Research Council Future Fellowship FT160100218, and R.S. received Computational Biology Research Initiative and Early Career Research Grants from the University of Melbourne. Y.W. was supported by a Natural Sciences and Engineering Research Council of Canada Postgraduate Scholarship D and a University of British Columbia Four-Year Fellowship Scholarship. J.L.H. was supported by the Center for Lignocellulose Structure and Formation and the Energy Frontier Research Center funded by the Department of Energy Office of Sciences, Basic Energy Sciences under Award DE-SC0001090.