Journal article

THE ROLE OF MICROFILAMENTS IN THE PRIMING EFFECT OF LH-RELEASING HORMONE - AN ULTRASTRUCTURAL-STUDY USING CYTOCHALASIN-B

CE LEWIS, JF MORRIS, G FINK

JOURNAL OF ENDOCRINOLOGY | J ENDOCRINOLOGY LTD | Published : 1985

Abstract

We have investigated the possibility that microfilaments are involved in the priming effect of LH-releasing hormone (LHRH) by ultrastructural morphometry of hemipituitary glands from adult female mice. Glands incubated for 2 consecutive hours with 8.5 nmol LHRH/l responded with a marked increase in the amount of LH released into the medium during the second hour compared with the first hour of incubation. This priming effect of LHRH on LH secretion was accompanied by a significant margination of secretory granules and a drop in the total granule content of the gonadotrophs. Although the number of microfilaments remained the same, there was an increase in their length and a change in orientat..

View full abstract