Journal article

PRIMING EFFECT OF LUTEINIZING-HORMONE RELEASING FACTOR - INVITRO AND INVIVO EVIDENCE CONSISTENT WITH ITS DEPENDENCE UPON PROTEIN AND RNA-SYNTHESIS

AJMC PICKERING, G FINK

JOURNAL OF ENDOCRINOLOGY | J ENDOCRINOLOGY LTD | Published : 1976

Abstract

The aim of this study was to determine whether the priming effect of LH-RF depends upon RNA and protein synthesis. In in-vivo studies saline, actinomycin D, or cycloheximide was administered i.p. 3-5-4h before the first i.v. injection of synthetic LH-RF into pro-oestrous rats anaesthetized with sodium pentobarbitone at 13.30 h. The LH-response to the second injection of LH-RF (given 60 min after the first) was markedly reduced by the inhibitors, but the response to the first injection was not significantly affected. Studies with cycloheximide given i.v. showed that the inhibition of protein synthesis up to the second injection of LH-RF reduced the magnitude of the priming effect, the reducti..

View full abstract