Journal article

ESTRADIOL-17BETA AND PITUITARY RESPONSIVENESS TO LUTEINIZING-HORMONE RELEASING FACTOR IN RAT - STUDY USING RECTANGULAR PULSES OF ESTRADIOL-17BETA MONITORED BY NON-CHROMATOGRAPHIC RADIOIMMUNOASSAY

SR HENDERSON, C BAKER, G FINK

JOURNAL OF ENDOCRINOLOGY | J ENDOCRINOLOGY LTD | Published : 1977

Abstract

Plasma concentrations of oestradiol-17beta were measured by a non-chromatographic radioimmunoassay during the oestrous cycle, after the s.c. injection of 2-5 or 10 microgram oestradiol benzoate (OB), or the s.c. implantation of Silastic capsules containing crystalline oestradiol-17beta. The profile of endogenous plasma oestradiol-17beta concentrations was similar to that reported by other workers, and lay between the concentrations produced by the low and high doses of OB. The rectangular pulses of increased plasma oestradiol concentrations, produced during the period of implantation of the Silastic capsules, were used to determine the time taken for oestradiol-17beta to exert its facilitato..

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