Journal article

THE VIP(2) RECEPTOR - MOLECULAR CHARACTERIZATION OF A CDNA-ENCODING A NOVEL RECEPTOR FOR VASOACTIVE-INTESTINAL-PEPTIDE

EM LUTZ, WJ SHEWARD, KM WEST, JA MORROW, G FINK, AJ HARMAR

FEBS Letters | ELSEVIER SCIENCE BV | Published : 1993

Abstract

We have cloned and sequenced a cDNA (RPR4) encoding a new member of the secretin/calcitonin/parathyroid hormone (PTH) receptor family. RPR4 was identified by PCR of rat pituitary cDNA, and a full-length clone was isolated from a rat olfactory bulb cDNA library. When RPR4 was functionally expressed in COS 7 cells, cyclic adenosine monophosphate (cAMP) production was stimulated by vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptides (PACAP-38 and PACAP-27) and helodermin, with equal potency. Peptide histidine isoleucine (PHI) and rat growth hormone releasing hormone (rGHRH) also stimulated cAMP production at lower potency. This suggests that RPR4 encodes a novel..

View full abstract