Journal article

Universal Alternative Splicing of Noncoding Exons

Ira W Deveson, Marion E Brunck, James Blackburn, Elizabeth Tseng, Ting Hon, Tyson A Clark, Michael B Clark, Joanna Crawford, Marcel E Dinger, Lars K Nielsen, John S Mattick, Tim R Mercer

CELL SYSTEMS | CELL PRESS | Published : 2018

Abstract

The human transcriptome is so large, diverse, and dynamic that, even after a decade of investigation by RNA sequencing (RNA-seq), we have yet to resolve its true dimensions. RNA-seq suffers from an expression-dependent bias that impedes characterization of low-abundance transcripts. We performed targeted single-molecule and short-read RNA-seq to survey the transcriptional landscape of a single human chromosome (Hsa21) at unprecedented resolution. Our analysis reaches the lower limits of the transcriptome, identifying a fundamental distinction between protein-coding and noncoding gene content: almost every noncoding exon undergoes alternative splicing, producing a seemingly limitless variety ..

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University of Melbourne Researchers

Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by NHMRC Australia Fellowship


Awarded by NHMRC Early Career Fellowship


Awarded by EMBO Long Term Fellowship


Awarded by Cancer Institute NSW Early Career Fellowship


Awarded by Novo Nordisk Fonden


Funding Acknowledgements

The authors acknowledge the following funding sources: an Australian National Health and Medical Research Council (NHMRC) Project grant (APP1062106 to T.R.M.), NHMRC Australia Fellowship (631668 to J.S.M.), an NHMRC Early Career Fellowship (APP1072662 to M.B.C.), an EMBO Long Term Fellowship (ALTF 864-2013 to M.B.C.), the Australian Research Council (Special Research Initiative in Stem Cell Science to L.K.N.), a Cancer Institute NSW Early Career Fellowship (2018/ECF013 to I.W.D.), and the generous support of the Paramor family (to T.R.M.). The contents of the published material are solely the responsibility of the administering institution, a participating institution, or individual authors and do not reflect the views of NHMRC or ARC. The authors thank the ENCODE consortium for the provision of data; data were employed in accordance with the data-release policy.