Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

Melissa E Carew; Rhys A Coleman; Ary A Hoffman

Journal article

Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

Melissa E Carew, Rhys A Coleman, Ary A Hoffman

PEERJ | PEERJ INC | Published : 2018

DOI: 10.7717/peerj.4980

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Abstract

Background: High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring. As metabarcoding becomes more widely applied, new reference DNA barcodes linked to individual specimens identified by taxonomists are needed. This can be achieved through using DNA extraction methods that are not only suitable for metabarcoding but also for building reference DNA barcode libraries. Methods: In this study, we test the suitability of a rapid non-destructive DNA extraction method for metabarcoding of freshwater invertebrate samples. Results: This method resulted in detection of taxa fro..

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University of Melbourne Researchers

Rhys Coleman Author Ecosystem and Forest Sciences

Ary Hoffmann Author Biosciences

Melissa Carew Author Biosciences

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

All funding for this study was from the Australian Research Council through their grant (grant number LP150100876) and Fellowship (grant number FL100100066), with additional support from Melbourne Water Corporation. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.