Journal article

Multiplexed Division Tracking Dyes for Proliferation-Based Clonal Lineage Tracing

Miles B Horton, Giulio Prevedello, Julia M Marchingo, Jie HS Zhou, Ken R Duffy, Susanne Heinzel, Philip D Hodgkin

The Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2018

Abstract

The generation of cellular heterogeneity is an essential feature of immune responses. Understanding the heritability and asymmetry of phenotypic changes throughout this process requires determination of clonal-level contributions to fate selection. Evaluating intraclonal and interclonal heterogeneity and the influence of distinct fate determinants in large numbers of cell lineages, however, is usually laborious, requiring familial tracing and fate mapping. In this study, we introduce a novel, accessible, high-throughput method for measuring familial fate changes with accompanying statistical tools for testing hypotheses. The method combines multiplexing of division tracking dyes with detecti..

View full abstract

Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Australian Government National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme


Awarded by Science Foundation Ireland


Awarded by European Union


Funding Acknowledgements

This work was supported by the National Health and Medical Research Council of Australia via Project Grant 1010654, Program Grant 1054925, and a fellowship to P.D.H., as well as Australian Government National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme Grant 361646. K.R.D. was supported by Science Foundation Ireland Grant 12IP1263. J.M.M. was the recipient of a Sydney Parker Smith Postdoctoral Research Fellowship from the Cancer Council of Victoria. J.H.S.Z. was the recipient of an Australian Postgraduate Award. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant Agreement 317040 (QuanTI).