Journal article

Zinc stress induces copper depletion in Acinetobacter baumannii

Karl A Hassan, Victoria G Pederick, Liam DH Elbourne, Ian T Paulsen, James C Paton, Christopher A McDevitt, Bart A Eijkelkamp

BMC MICROBIOLOGY | BIOMED CENTRAL LTD | Published : 2017

Abstract

BACKGROUND: The first row transition metal ions zinc and copper are essential to the survival of many organisms, although in excess these ions are associated with significant toxicity. Here, we examined the impact of zinc and copper stress on Acinetobacter baumannii, a common opportunistic pathogen. RESULTS: We show that extracellular zinc stress induces a copper-specific depletion phenotype in A. baumannii ATCC 17978. Supplementation with copper not only fails to rescue this phenotype, but further exacerbates the copper depletion. Extensive analysis of the A. baumannii ATCC 17978 genome identified 13 putative zinc/copper resistance efflux pumps. Transcriptional analyses show that four of th..

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Grants

Awarded by National Health and Medical Research Council (Australia)


Awarded by KAH


Awarded by Channel 7 Children's Research Foundation


Awarded by LDHE by a Macquarie University Research Development


Awarded by Australian Research Council Discovery Project


Funding Acknowledgements

This work was supported by the National Health and Medical Research Council (Australia) through Project Grant 1060895 to ITP and KAH, Project Grant 1080784 to CAM and Program Grant 1071659 to JCP. BAE and CAM are supported by Channel 7 Children's Research Foundation Grant 161203, and KAH and LDHE by a Macquarie University Research Development Grant 9201401563. The work was also funded by the Australian Research Council Discovery Project Grants DP120101432 to JCP, and DP170102102 and DP150101856 to JCP and CAM. JCP is a NHMRC Senior Principal Research Fellow.