A sandwich enzyme-linked immunosorbent assay for the quantitation of human plasma ferritin

AV Bleicher; HW Unger; SJ Rogerson; EH Aitken

Journal article

A sandwich enzyme-linked immunosorbent assay for the quantitation of human plasma ferritin

AV Bleicher, HW Unger, SJ Rogerson, EH Aitken

Methodsx | Published : 2018

DOI: 10.1016/j.mex.2018.06.010

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Abstract

There is a lack of published enzyme linked immunosorbent assay (ELISA) protocols which use commercially available reagents for the measurement of ferritin in human plasma for research purposes. ELISA kits are often expensive and do not always provide detailed information about reagents included. A commercially available antibody pair was used to develop an in-house ELISA to measure ferritin in small (25 μl) volumes of human plasma. ELISA results were compared to ferritin levels measured using a commercial immune-assay system. The sensitivity, intra and inter assay variation of the ELISA assay were also determined. ELISA measurements of plasma ferritin ranged between 3.2–232 ng/mL and were co..

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University of Melbourne Researchers

Holger Unger Author

Stephen Rogerson Author

Elizabeth Aitken Author

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Funding Acknowledgements

We would like to thank all the individuals who donated plasma samples and Maria Bisignano who measured ferritin on a subset of samples at the Melbourne Health Shared Pathology Service. The samples used were originally collected using funding from the Malaria in Pregnancy Consortium which is supported by the Bill & Melinda Gates Foundation. This work was funded by a grant from the British Maternal and Fetal Medicine Society to HWU.