Journal article

Biotin-mediated growth and gene expression in Staphylococcus aureus is highly responsive to environmental biotin

Jiulia Satiaputra, Bart A Eijkelkamp, Christopher A McDevitt, Keith E Shearwin, Grant W Booker, Steven W Polyak



Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates expression of biotin biosynthetic enzymes in response to biotin demand and supply. Here, we compare the effect of biotin on the growth of two bacteria that possess a bifunctional BirA, namely Escherichia coli and Staphylococcus aureus. Unlike E. coli that could fulfill its biotin requirements through de novo synthesis, S. aureus showed improved growth rates in media supplemente..

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Awarded by National Health and Medical Research Council of Australia

Awarded by Australian Research Council

Funding Acknowledgements

This study was funded by the National Health and Medical Research Council of Australia Project Grant 1068885 awarded to SWP and GWB and Project Grants 1080784 and 1122582 awarded to CAM, and the Australian Research Council Discovery Project DP160101450 awarded to KES and DP150101856 and DP170102102 awarded to CAM. JS is a recipient of The University of Adelaide faculty divisional scholarship.