Journal article
Hybrid Conjugates Formed between Gold Nanoparticles and an Amyloidogenic Diphenylalanine-Cysteine Peptide
JNBD Pelin, E Gatto, M Venanzi, F Cavalieri, CLP Oliveira, H Martinho, ER Silva, AM Aguilar, JS Souza, WA Alves
Chemistryselect | WILEY-V C H VERLAG GMBH | Published : 2018
Abstract
We investigated the effect of gold nanoparticles (AuNPs) on the aggregation of a CFF (C=cysteine; F=phenylalanine) tripeptide (derived from Aβ peptide) in aqueous medium. Special attention was dedicated to the role of AuNPs as inducers and inhibitors during nucleation kinetics and the structure of the resulting scaffolds was carefully investigated. At millimolar concentrations, the tripeptide was found to form β-sheet structures organized into long filaments. Spectral signatures and topography of the filaments were studied by Raman spectroscopy and atomic force microscopy (AFM), revealing that conjugation to AuNPs not only stabilizes the system, but also inhibit or enhance amyloid-like featu..
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Awarded by Horizon 2020 Framework Programme
Funding Acknowledgements
This work was supported by FAPESP (grant nos. 2015/24018-1, 2017/02317-2 and 2016/24409-3) and CNPq (grant no. 302923/2015-2). INCT in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7) is kindly acknowledged for the grants. J.N.B.D.P. acknowledges FAPESP (project number 2015/20446-9) for a doctoral fellowship. This project has also received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 690901. The staff at LNNano and LNLS are kindly recognized for invaluable help and access to AFM, TEM, SAXS facilities, respectivelly. The authors are grateful to the Multiuser Central Facilities (UFABC), Nanomedicine Research Strategic Unit (NANOMED) at UFABC and Instituto de Fisica - USP for the experimental support. C.L.P.O. is supported by the National Council for Scientific and Technological Development, the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx), and the Sao Paulo Research Foundation.