Derivation of phenotypically diverse neural culture from hESC by combining adherent and dissociation methods
Ye Liu, Ana Antonic, Xuan Yang, Nils Korte, Katherine Lim, Anna E Michalska, Mirella Dottori, David W Howells
JOURNAL OF NEUROSCIENCE METHODS | ELSEVIER | Published : 2018
BACKGROUND: Differentiation of human embryonic stem cells (hESCs) into distinct neural lineages has been widely studied. However, preparation of mixed yet neurochemically mature populations, for the study of neurological diseases involving mixed cell types has received less attention. NEW METHOD: We combined two commonly used differentiation methods to provide robust and reproducible cultures in which a mixture of primarily GABAergic and Glutamatergic neurons was obtained. Detailed characterisation by immunocytochemistry (ICC) and quantitative real-time PCR (qPCR) assessed the neurochemical phenotype, and the maturation state of these neurons. RESULTS: We found that once neurospheres (NSs) h..View full abstract
This work was supported by fund from "Biomarkers for assessing effectiveness of hypothermia as a therapy in ischaemic stroke" (grant number: 1037863).