Journal article

Microparticulate Caspase 1 Regulates Gasdermin D and Pulmonary Vascular Endothelial Cell Injury

Srabani Mitra, Matthew Exline, Fabien Habyarimana, Mikhail A Gavrilin, Paul J Baker, Seth L Masters, Mark D Wewers, Anasuya Sarkar

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY | AMER THORACIC SOC | Published : 2018

Abstract

Lung endothelial cell apoptosis and injury occur throughout all stages of acute lung injury/acute respiratory distress syndrome and impact disease progression. Caspases 1, 4, and 5 are essential for completion of the apoptotic program known as pyroptosis that also involves proinflammatory cytokines. Because gasdermin D (GSDMD) mediates pyroptotic death and is essential for pore formation, we hypothesized that it might direct caspase 1-encapsulated microparticle (MP) release and mediate endothelial cell death. Our present work provides evidence that GSDMD is released by LPS-stimulated THP-1 monocytic cells, where it is packaged into microparticles together with active caspase 1. Furthermore, ..

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University of Melbourne Researchers