Single Cell RNA Sequencing of stem cell-derived retinal ganglion cells
Maciej Daniszewski, Anne Senabouth, Quan Nguyen, Duncan Crombie, Samuel Lukowski, Tejal Kulkarni, Donald Zack, Alice Pébay, Joseph Powell, Alex Hewitt
Published : 2017
ABSTRACT We used human embryonic stem cell-derived retinal ganglion cells (RGCs) to characterize the transcriptome of 1,174 cells at the single cell level. The human embryonic stem cell line BRN3B-mCherry A81-H7 was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and subsequently prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells upregulated genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature phenotype.