Journal article
Isolation of Mycobacterium avium subspecies paratuberculosis from Ugandan cattle and strain differentiation using optimised DNA typing techniques
JB Okuni, CI Dovas, P Loukopoulos, IG Bouzalas, DP Kateete, ML Joloba, L Ojok
BMC Veterinary Research | BMC | Published : 2012
Abstract
Background: The occurrence of paratuberculosis in Ugandan cattle has recently been reported but there is no information on the strains of Mycobacterium avium subspecies paratuberculosis (MAP) responsible for the disease. The aim of this study was to isolate and characterise MAP from seropositive cattle and paratuberculosis lesions in tissues obtained from slaughtered cattle in Uganda.Results: Twenty one isolates of MAP were differentiated into 11 genotype profiles using seven genotyping loci consisting of Insertion Sequence 1311(IS1311), Mycobacterial interspersed repeat units (MIRU) (loci 2, 3), Variable number tandem repeats (VNTR) locus 32 and Short sequence repeats (SSR) (loci 1, 2 and 8..
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Funding Acknowledgements
The authors acknowledge financial assistance from Carnegie Corporations, New York; Makerere University and Aristotle University of Thessaloniki and the Coimbra scholarship committee for granting a scholarship to JBO to conduct part of the study at Aristotle University of Thessaloniki. We thank the management of the National leprosy and TB laboratories (Uganda) for providing access to culture facilities. Special thanks go to Prof. Raul Barletta of the University of Nebraska-Lincoln for providing control DNA from MAP strain K10. We thank Mr. Musisi Joseph and other the field Veterinary staff in the different districts who assisted during field work.