Establishment of an animal model for Waldenström's macroglobulinemia

AS Tsingotjidou; CE Emmanouilides; E Siotou; T Poutahidis; A Xagorari; P Loukopoulos; D Sotiropoulos; C Bekiari; M Doulberis; P Givissis; A Fassas; A Anagnostopoulos

Journal article

Establishment of an animal model for Waldenström's macroglobulinemia

AS Tsingotjidou, CE Emmanouilides, E Siotou, T Poutahidis, A Xagorari, P Loukopoulos, D Sotiropoulos, C Bekiari, M Doulberis, P Givissis, A Fassas, A Anagnostopoulos

Experimental Hematology | ELSEVIER SCIENCE INC | Published : 2009

DOI: 10.1016/j.exphem.2008.12.007

Abstract

Objective: Waldenström's macroglobulinemia (WM) is a low-grade lymphoplasmacytoid lymphoma characterized by production of monoclonal immunoglobulin M (IgM). The present study was undertaken with the aim of developing a novel nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse model of WM. Materials and Methods: Pairs of bone particles derived from adult humans were successfully implanted intramuscularly in NOD/SCID mice. Each mouse was implanted with a bone fragment taken from a neoplastic disease-free individual in the one hind limb and with a different biopsy taken from a WM patient in the other. Results: All mice implanted with the bone marrow core biopsies had increased le..

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