Journal article
Acoustically-mediated intracellular delivery
Shwathy Ramesan, Amgad R Rezk, Chaitali Dekiwadia, Christina Cortez-Jugo, Leslie Y Yeo
Nanoscale | Royal Society of Chemistry | Published : 2018
DOI: 10.1039/c8nr02898b
Abstract
Recent breakthroughs in gene editing have necessitated practical ex vivo methods to rapidly and efficiently re-engineer patient-harvested cells. Many physical membrane-disruption or pore-forming techniques for intracellular delivery, however, result in poor cell viability, while most carrier-mediated techniques suffer from suboptimal endosomal escape and hence cytoplasmic or nuclear targeting. In this work, we show that short exposure of cells to high frequency (>10 MHz) acoustic excitation facilitates temporal reorganisation of the lipid structure in the cell membrane that enhances translocation of gold nanoparticles and therapeutic molecules into the cell within just ten minutes. Due to it..
View full abstractGrants
Awarded by Australian Research Council (ARC)
Awarded by ARC
Awarded by Australian Research Council
Funding Acknowledgements
ARR is grateful for an RMIT University Vice-Chancellor's Postdoctoral Fellowship and LYY for an Australian Research Council (ARC) Future Fellowship (FT130100672). LYY also acknowledges funding from the ARC through Discovery Project (DP170101061). The authors thank Dr Zeyad Nasa for technical assistance with the use of the facilities at the RMIT MicroNano Research Facility.