Journal article
Nanosized, peptide-based multicomponent DNA delivery systems: Optimization of endosome escape activity
Y Wan, PM Moyle, MP Christie, I Toth
Nanomedicine | FUTURE MEDICINE LTD | Published : 2016
DOI: 10.2217/nnm.16.27
Abstract
Aim: Endosome escape is essential for developing effective nonviral gene delivery systems. Herein, three endosome-disrupting peptides (HA2(1-20), GALA and KALA) were incorporated into a multicomponent oligonucleotide delivery system to identify which peptide imparted the most favorable endosome escape and toxicity profile. Materials & methods: Copper (I)-catalyzed azide-alkyne cycloaddition was used to construct multicomponent delivery vectors. The systems were evaluated for size, toxicity, cellular uptake and endosome escape activity. Results: Each system condensed plasmid DNA to form nanosized particles. The highest cellular uptake and endosome escape were associated with GALA and KALA con..
View full abstractGrants
Awarded by Australian Research Council (ARC)
Awarded by NHMRC postdoctoral training fellowship
Awarded by ARC Australian Professorial Fellowship
Funding Acknowledgements
This work was supported by Australian Research Council (ARC) grant DP130100952. Y Wan, PM Moyle and I Toth were supported by a University of Queensland International Postgraduate Research Scholarship, NHMRC postdoctoral training fellowship (569869) and ARC Australian Professorial Fellowship (DP110100212), respectively. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.