Journal article
Peptide based DNA nanocarriers incorporating a cell-penetrating peptide derived from neurturin protein and poly-l-lysine dendrons
N Rosli, MP Christie, PM Moyle, I Toth
Bioorganic and Medicinal Chemistry | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2015
Abstract
Multicomponent gene delivery systems incorporating cell-penetrating peptides (CPP) from the human neurturin protein (NRTN-30, NRTN(132-161); NRTN-17, NRTN(145-161)) and a poly-l-lysine (PLL) dendron, were synthesized and characterized for plasmid DNA (pDNA) delivery. Acetylated NRTN peptides (Ac-CPP) and peptides conjugated to a PLL dendron (DEN-CPP) efficiently condensed and stabilized pDNA. Complexes between pDNA and DEN-CPP formed smaller and more stable nanoparticles. Flow cytometry experiments showed that pDNA-DEN-CPPs were taken up more efficiently into HeLa cells. There was also no significant difference between NRTN-30 and NRTN-17 for pDNA uptake, indicating that the truncated peptid..
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Awarded by Australian Research Council
Funding Acknowledgements
The authors would like to acknowledge the technical support of Dr Michael Nefedov for flow cytometry, and Dr Steven Mason for confocal imaging. This work was supported by an Australian Research Council Discovery Project grant (DP130100952) and a Professorial Research Fellowship (DP110100212) to IT. PMM received fellowship funding from the Australian National Health and Medical Research Council (NHMRC; 569869). The funding bodies were not involved in the design of the study, data collection, analysis and interpretation, preparation of the manuscript or in the decision to submit the manuscript for publication.