Journal article
Multiplex immunohistochemistry accurately defines the immune context of metastatic melanoma
H Halse, AJ Colebatch, P Petrone, MA Henderson, JK Mills, H Snow, JA Westwood, S Sandhu, JM Raleigh, A Behren, J Cebon, PK Darcy, MH Kershaw, GA McArthur, DE Gyorki, PJ Neeson
Scientific Reports | NATURE PORTFOLIO | Published : 2018
Open access
Abstract
A prospective study explored the heterogeneous nature of metastatic melanoma using Multiplex immunohistochemistry (IHC) and flow cytometry (FACS). Multiplex IHC data quantitated immune subset number present intra-tumoral (IT) vs the tumor stroma, plus distance of immune subsets from the tumor margin (TM). In addition, mIHC showed a close association between the presence of IT CD8+ T cells and PDL1 expression in melanoma, which was more prevalent on macrophages than on melanoma cells. In contrast, FACS provided more detailed information regarding the T cell subset differentiation, their activation status and expression of immune checkpoint molecules. Interestingly, mIHC detected significantly..
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Awarded by Victorian Cancer Agency
Funding Acknowledgements
Funding for this study was provided by PeterMac Foundation and the Melbourne Melanoma Project (Victorian Cancer Agency). The authors wish to acknowledge the PeterMac Melanoma Biomarker Study for facilitating access to the melanoma samples from patients in this study, Dr Sarah Ellis (Center for advanced histology and microscopy, Peter MacCallum Cancer Centre) for creating the distance algorithm used in Figure 1, and the FACS facility (Ralph Rossi, Viki Milovac, Sophie Curcio) for their support. We also wish to thank the Ian Potter Foundation for a grant (20150678) to purchase the Vectra system. Finally, we wish to thank Minyu Wang and Han Aw Yeang for creating H&E and mIHC images.