Multiplex immunohistochemistry accurately defines the immune context of metastatic melanoma
H Halse, AJ Colebatch, P Petrone, MA Henderson, JK Mills, H Snow, JA Westwood, S Sandhu, JM Raleigh, A Behren, J Cebon, PK Darcy, MH Kershaw, GA McArthur, DE Gyorki, PJ Neeson
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2018
Awarded by Ian Potter Foundation
Funding for this study was provided by PeterMac Foundation and the Melbourne Melanoma Project (Victorian Cancer Agency). The authors wish to acknowledge the PeterMac Melanoma Biomarker Study for facilitating access to the melanoma samples from patients in this study, Dr Sarah Ellis (Center for advanced histology and microscopy, Peter MacCallum Cancer Centre) for creating the distance algorithm used in Figure 1, and the FACS facility (Ralph Rossi, Viki Milovac, Sophie Curcio) for their support. We also wish to thank the Ian Potter Foundation for a grant (20150678) to purchase the Vectra system. Finally, we wish to thank Minyu Wang and Han Aw Yeang for creating H&E and mIHC images.