Journal article
Low levels of HIV-1 envelope-mediated fusion are associated with long-Term survival of an infected CCR5S/S patient
PR Gorry, F Ahmad, J Mohl, G Alkhatib
AIDS | LIPPINCOTT WILLIAMS & WILKINS | Published : 2018
Abstract
Objectives: This study investigated whether Env-mediated fusion levels of R5X4 viruses are associated with long-Term survival of an infected CCR5 patient. Design: Four R5X4 Envs were cloned from each of two infected homosexual individuals (DR and C2) homozygous for the CCR5D32 allele. DR is a long-Term survivor chronically infected with HIV-1 and his Envs were cloned 12 years after testing HIVinfected, whereas C2 Envs were isolated 1 year after primary infection. Methods: The current study sequenced the gp41 subunits and created hybrid Envs that contained exchanged gp41 subunits or V3 loops. The Env-mediated fusion activity of Envs was examined in cell fusion and virus infection assays. Resu..
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Awarded by National Institutes of Health
Funding Acknowledgements
[ "We thank Dr Chris Broder for providing T50 and other monoclonal antibodies to the gp120 protein. Special thanks go to Andy Grass, Viswanathan Rajagopalan, Troy Camarata, and Carol Brenner for excellent comments. The following reagents were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: T20, Fusion Inhibitor from AIDS, NIAID; HIV-1 gp160 Antiserum (HT7); 3T3. T4, 3T3. T4. CXCR4, and 3T3. T4. CCR5 from Dr Dan Littman. This work was supported by a NIH grant # AI052019 awarded to GA.", "This work was supported by a NIH grant # AI052019 awarded to GA." ]