Journal article

Proteomic diversity in a prevalent human-infective Giardia duodenalis sub-species

Samantha J Emery-Corbin, Daniel Vuong, Ernest Lacey, Staffan G Svard, Brendan RE Ansell, Aaron R Jex



Giardia duodenalis a species complex of gastrointestinal protists, with assemblages A and B infective to humans. To date, post-genomic proteomics are largely derived from Assemblage A, biasing understanding of parasite biology. To address this gap, we quantitatively analysed the proteomes of trophozoites from the genome reference and two clinical Assemblage B isolates, revealing lower spectrum-to-peptide matches in non-reference isolates, resulting in significant losses in peptide and protein identifications, and indicating significant intra-assemblage variation. We also explored differential protein expression between in vitro cultured subpopulations putatively enriched for dividing and fee..

View full abstract


Awarded by Australian Research Council (ARC)

Awarded by NHMRC

Awarded by Jack Brockhoff Foundation Early Career Grant (Australia)

Funding Acknowledgements

This work, including the efforts of AJ, was funded by the Australian Research Council (ARC) (LP120200122). SE, BA and AJ are supported by the Victorian State Government Operational Infrastructure Support (Australia) and Australian Government National Health and Medical Research Council (NHMRC) Independent Research Institute Infrastructure Support Scheme. AJ is also supported by a NHMRC Career Development Fellowship (APP1126395). SE and this research are also supported by a Jack Brockhoff Foundation Early Career Grant (Australia) (ID JBF 4184, 2016). Proteomic analysis was performed at the Mass Spectrometry and Proteomics Facility at Bio21 at Melbourne University (Australia) with assistance from Ching-Seng Ang and Shuai Nie. The authors also wish to acknowledge Professor Jacqui Uperoft (Australia) for providing the in vitro Giardia isolates used in this study. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.